HOME > mice > Glb1E6-KO mice
Strain Detail: Glb1E6-KO mice
| Strain information |
| Resource No. |
nbio417 |
| Strain name |
Glb1E6-KO |
| Strain Nomenclature |
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| Common name/Synonyms |
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| Strain types |
mutant |
| Background strain |
C57BL/6N |
| Institution |
Laboratory of Animal Models for Human Diseases, National Institutes of Biomedical Innovation, Health and Nutrition |
| Developer |
Osamu Suzuki |
| Depositor |
Osamu Suzuki |
| Conditions of distribution |
Collaboration with the depositor only. It's necessary to obtain depositor's consent on use of biological resources. contact us |
| Animal Health Report |
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| Strain description |
 Our laboratory has used knockout (KO) mice (BKO) of the Glb1 gene as a mouse model for GM1 gangliosidosis. However, BKO mice have some drawbacks, such as the expression of an unwanted gene (Neo resistance gene), so we generated new KO mice by deleting exon 6 in the B6N background by genome editing. The loss of the GLB1 protein was confirmed (see WB Fig.) and, this strain is expected to be used as a mouse model for GM1 gangliosidosis like BKO. |
| Reference(s) |
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| Resource information |
| Cryopreserved sperm |
In-house |
Genotype |
Heterozygous |
| Medium |
Fertiup |
| From other institutions |
Genotype |
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| Medium |
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| Cryopreserved embryo |
In-house |
Mating System |
Heterozyous x B6NCr Slc |
| Genotype |
Heterozygous or wild |
| Medium |
EFS40 |
| From other institutions |
Mating System |
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| Genotype |
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| Method |
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| Strain status / Availability |
Cryopreserved sperm |
Within 1 month |
| Cryopreserved embryo |
Within 1 month |
| Live animals |
Approx. 2 months |
| Gene information |
| Mutation types |
knockout |
| Gene symbol |
Glb1 |
| Gene name |
galactosidase, beta 1 |
| Genotyping information |
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