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Strain Detail: AMPAM

Strain information
Resource No. nbio275
Strain name AMPAM
Strain Nomenclature B6-Tg(Eevee-AMPK) pT2A-lox-tdKeima-4031NES
Common name/Synonyms
Strain types mutant
Background strain C57BL/6J
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

The AMPAM mice are transgenic mice expressing a cytoplasm-localized förster (or fluorescence) resonance energy transfer (FRET) biosensor for monitoring AMPK kinase activity. The AMPAM mice were derived from transgenic expressing a probe with both lox-tdKeima and FRET sensor. The lox-tdKeima in the probe was removed by mating with Ella-Cre mice, and therefore, the AMPAM mice express the FRET biosensor in the whole body. AMPK (AMP-activated protein kinase) is a protein kinase, which phosphorylates various proteins in the presence of AMP. It promotes ATP-producing pathways and inhibits ATP-consuming biosynthetic processes, including gluconeogenesis, lipid and protein synthesis. Through these processes, AMPK suppresses the proliferation of cells like tumor cells. With this strain, AMPK activity can be measured spatially and temporally in various tissues of fetuses and adults by live image observation of this strain under the two-photon microscopy. This strain is useful not only for analysis of the signal transduction cascade in live animals, but also for the application of drug development, such as the pharmacodynamic analysis targeting signal molecules, because rich expression of FRET biosensors can be observed even in primary cultured cells derived from various tissues.

References
Resource information
Cryopreserved sperm In-house Genotype Tg/+
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System IVF
Genotype mix of heterozygous transgenic and wild-type
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol CFP, YFP, tdKeima
Gene name Cyan Fluorescent Protein, Yellow Fluorescent Protein, tandem dimer of Keima
Genotyping information Presence of transgenes can be checked by the emission of light green fluorescence (~530 nm, YFP’s fluorescence) from the surface of mouse bodies under the blue excitation light (~450 nm).