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Strain Detail: AMPK-CA/B mice
| Strain information | |
|---|---|
| Resource No. | nbio089 |
| Strain name | AMPK-CA/B |
| Strain Nomenclature | |
| Common name/Synonyms | |
| Strain types | mutant |
| Background strain | |
| Institution | Division of basic nutrition, National Institute of Health and Nutrition |
| Developer | Shinji Miura |
| Depositor | Shinji Miura |
| Conditions of distribution | Only for collaboration with the depositor. contact us |
| Animal Health Report | |
| Strain description | AMPK-CA/B mice are transgenic mice specifically overexpressing rat constitutive active AMPK α1 subunit in skeletal muscle. This strain expresses transgene introduced T172D mutation into cDNA encoding amino acids 1-312 of rat AMPK α1 subunit under the control of human α actin promoter on -2,000 bp. However, these mice didn't observe to increase AMPK activity in skeletal muscle. AMPK is an enzyme phosphorylating protein in the presence of AMP, and it's hetero trimer constructed with three subunit, α, β and γ. This strain is availabale for research about obesity and diabetes because AMPK regulates enzyme activity related to carbohydrate or lipid metabolism. This strain has 4 lines. |
| Reference(s) | |
| Resource information | |||
|---|---|---|---|
| Cryopreserved sperm | In-house | Genotype | |
| Medium | FERTIUP | ||
| From other institutions | Genotype | ||
| Medium | |||
| Cryopreserved embryo | In-house | Mating System | |
| Genotype | |||
| Medium | EFS40 | ||
| From other institutions | Mating System | ||
| Genotype | |||
| Method | |||
| Strain status / Availability | Cryopreserved sperm | Within 1 month | |
| Cryopreserved embryo | Within 1 month | ||
| Live animals | Approx. 2 months | ||
| Gene information | |
|---|---|
| Mutation types | transgenic |
| Gene symbol | Ampk |
| Gene name | AMP-activated protein kinase |
| Genotyping information | |