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Strain Detail: AMPK-DN/D mice

Strain information
Resource No. nbio086
Strain name AMPK-DN/D
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain
Institution Division of basic nutrition, National Institute of Health and Nutrition
Developer Shinji Miura
Depositor Shinji Miura
Conditions of distribution

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Animal Health Report
Strain description

AMPK-DN/D mice are transgenic mice specifically overexpressing rat dominant negative AMPK α1 subunit in skeletal muscle. This strain expresses transgene introduced D157A mutation into cDNA encoding full length amino acid sequence of rat AMPK α1 subunit under the control of human α actin promoter on -2,000 bp. These mice decreased AMPK α1 subunit activity to 50% in skeletal muscle and almost lost α2 subunit activity completely. AMPK is an enzyme phosphorylating protein in the presence of AMP, and it's hetero trimer constructed with three subunit, α, β and γ. This strain is availabale for research about obesity and diabetes because AMPK regulates enzyme activity related to carbohydrate or lipid metabolism. This strain has 6 lines.
Reference(s)
  1. Miura S, Kai Y, Kamei Y, Bruce CR, Kubota N, Febbraio MA, Kadowaki T, and Ezaki O. (2009) α2-AMPK activity is not essential for an increase in fatty acid oxidation during low-intensity exercise. Am J Physiol Endocrinol Metab 296(1):E47-55. [PMID:18940938]
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol Ampk
Gene name AMP-activated protein kinase
Genotyping information