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Strain Detail: C57BL/6N.129/SvJ-Slc3a2tm1

Strain information
Resource No. nbio305
Strain name C57BL/6N.129/SvJ-Slc3a2tm1
Strain Nomenclature
Common name/Synonyms CD98 flox mice
Strain types mutant
Background strain
Institution Division of Laboratory Animal Resources, National Research Institute for Child Health and Development
Developer Hideki Tsumura
Depositor Hideki Tsumura
Conditions of distribution

It's necessary to obtain depositor's consent on use of biological resources. Contact us. Users of this strain have to include the depositor as a co-author in users' first scientific paper using this strain.

Animal Health Report
Strain description

CD98, aka 4F2 antigen, is a protein composed of heavy (CD98hc, Slc3a2) and light chains. CD98 is involved in various activities, such as amino acid transport, lymphocyte activation, integrin regulation, and fusion regulation. Since CD98hc deficient mice are embryonic lethal (Ref. 1), this conditional knockout mouse strain was produced with floxed exon 3 of Slc3a2 gene (Ref. 2). Researchers can induce conditional KO in the Slc3a2 gene by crossing mice with Cre recombinase expression. This strain was derived from ES cells with 129 background (EW4 cell line) and then backcrossed to C57BL/6N mice (N=18 at introduction into NIBIOHN). This strain is useful for studying CD98hc functions in various tissues.

References
  1. Tsumura H, Suzuki N, Saito H, Kawano M, Otake S, Kozuka Y, Komada H, Tsurudome M, and Ito Y. (2003) The targeted disruption of the CD98 gene results in embryonic lethality. Biochem. Biophys. Res. Commun. 308(4):847-51. [PMID:12927796]
  2. Tsumura H, Ito M, Li XK, Nakamura A, Ohnami N, Fujimoto J, Komada H, and Ito Y. (2012) The role of CD98hc in mouse macrophage functions. Cell. Immunol. 276(1-2):128-34. [PMID:22624878]
Resource information
Cryopreserved sperm In-house Genotype Heterozygous
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System IVF
Genotype mix of KO/+ and wild-type
Medium EFS40
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types conditional knockout
Gene symbol Slc3a2 (CD98hc)
Gene name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Genotyping information