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Strain Detail: B6N lox-Booster-PKAchu mice

Strain information
Resource No. nbio371
Strain name B6N lox-Booster-PKAchu
Strain Nomenclature
Common name/Synonyms lox-Booster-PKAchu
Strain types mutant
Background strain C57BL/6N
Institution Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. contact us
Animal Health Report
Strain description

Transgenic mice expressing a probe with lox-mCherry-NLS added upstream of a cytoplasmically-localized FRET biosensor (Booster-PKA) that monitors the kinase activity of PKA. The mice with mCherry-NLS recombination induced by crossing with EIIa-Cre (+/+) mice systemically express Booster-PKA. Background strain heterozygous for the Tyrc gene is a hybrid between wild-type B6N and B6N Albino mice.

  1. Watabe T, Terai K, Sumiyama K, Matsuda M. (2020) Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools. ACS Sens. 5:719-730. [PMID: 32101394]
Resource information
Cryopreserved sperm In-house Genotype
From other institutions Genotype heterozygous
Cryopreserved embryo In-house Mating System
From other institutions Mating System
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo
Live animals Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol mCherry, mKate2, mKOκ
Gene name red, red, and orange fluorescent proteins.
Genotyping information