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Strain Detail: Balb/c.Eisuke-NES mice

Strain information
Resource No. nbio284
Strain name Balb/c.Eisuke-NES
Strain Nomenclature Balb/c-Tg(EKAREV)pT2A-3903NES
Common name/Synonyms
Strain types mutant
Background strain BALB/c
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. :contact us
Animal Health Report
Strain description

The Balb/c.Eisuke-NES mice are transgenic mice with BALB/c background expressing cytoplasm-localized förster (or fluorescence) resonance energy transfer (FRET) biosensor EKAREV-NES monitoring ERK kinase activity under the control of CAG promoter in the whole body. Extracellular signal-regulated kinases (ERK) belonging to MAPK family are involved in the cellular growth and differentiation, especially play important roles in oncogene signal transduction cascade. It is possible to measure ERK kinase activity spatially and temporally in fetal or adult various tissues by live imaging observation of this strain in the two-photon microscopy. This strain expects not only to analyze the signal transduction cascade in live animals, but also to apply drug development such as the pharmacodynamic analysis targeting signal molecules because these mice show rich expression in primary cultured cells derived from various tissues. Cytoplasm-localized FRET biosensors are useful for the observation of cell shapes, whereas nucleus-localized FRET biosensors are better choices for comparison of ERK activity among cell lines derived from epithelial tissues. FRET biosensors generally can't be used for fixed samples.

Resource information
Cryopreserved sperm In-house Genotype Hemizygous
From other institutions Genotype
Cryopreserved embryo In-house Mating System
From other institutions Mating System
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo
Live animals Approx. 3 months
Gene information
Mutation types transgenic
Gene symbol CFP, YFP
Gene name Cyan Fluorescent Protein, Yellow Fluorescent Protein
Genotyping information The presence of transgenes can be confirmed by fluorescence (around 500 nm) from the surface of the mouse body under blue light (around 450 nm).