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Strain Detail: Balb/c.PKAchu mice

Strain information
Resource No. nbio283
Strain name Balb/c.PKAchu
Strain Nomenclature Balb/c-Tg(AKAR3EV)pT2A-3901NES
Common name/Synonyms
Strain types mutant
Background strain BALB/c
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. :contact us
Animal Health Report
Strain description

The Balb/c.PKAchu mice are congenic mice of PKAchu mice (nbio185) with the BALB/c genetic background. PKAchu mice are transgenic mice expressing cytoplasm-localized förster (or fluorescence) resonance energy transfer (FRET) biosensor AKAR3EV monitoring PKA kinase activity under the control of CAG promoter in the whole body. Protein kinases A (PKA, also known as the cyclic AMP-dependent protein kinases) are activated by the elevated intracellular cAMP in response to stimuli from hormone or bioactive substance, and relay signal transductions from hormones. With the biosensor, PKA kinase activity can be measured spatially and temporally in various fetal and adult tissues by the live-image observation of this strain under the two-photon microscopy. This strain is useful not only for analyses of the signal transduction cascade in live animals, but also for drug development such as the pharmacodynamic analysis targeting signal molecules, because these mice show rich expression in primary cultured cells derived from various tissues. In the developmental stage, biosensor expression was observable in most cells. FRET biosensors are not applicable for fixed samples.

Reference(s)
Resource information
Cryopreserved sperm In-house Genotype Hemizygous
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo
Live animals Approx. 3 months
Gene information
Mutation types transgenic
Gene symbol CFP, YFP
Gene name Cyan Fluorescent Protein, Yellow Fluorescent Protein
Genotyping information The presence of transgenes can be confirmed by fluorescence (around 500 nm) from the surface of the mouse body under blue light (around 450 nm).