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Strain Detail

Strain information
Resource No. nbio237
Strain name Bruce
Strain Nomenclature B6C3F1-Tg(pT2A-lox-tdKeima-FHA1-TQCHK2subs)
Common name/Synonyms
Strain types mutant
Background strain B6C3F1
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

The Bruce mice are transgenic mice expressing a probe consisting of lox-tdKeima and cytoplasm-localized föster (or fluorescence) resonance energy transfer (FRET) biosensor monitoring activity of an enzyme, ataxia telangiectasia mutated (ATM). ATM is a serine/threonine kinase induced by, e.g., DNA damage. The enzyme is involved in the check point mechanism of the cell cycle. Abnormality of ATM is related to tumorigenesis. The Bruce mice show CAG-promoter-driven systemic expression of Keima fluorescent proteins. By crossing Bruce mice with mice expressing tissue-specific Cre enzyme, tissue-specific switch of expression can be induced from Keima fluorescent protein to FRET biocensors. Observation under the two-photon microscope can visualize temporal and spatial changes of the ATM activity in various tissues of fetus and adult mice. This strain is expected not only for analysis of the signal transduction cascade in live animals, but also for drug development such as the pharmacodynamic analysis targeting signal molecules, because primary cultured cells derived from various tissues of the mice also show rich expression of FRET biocensors.

References
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol YFP, CFP, tdKeima
Gene name Yellow Fluorescent Protein, Cyan Fluorescent Protein, tandem dimer of Keima
Genotyping information