HOME > mice > Cmah-KO(129) mice

Strain Detail: Cmah-KO(129)

Strain information
Resource No. nbio261
Strain name Cmah-KO(129)
Strain Nomenclature
Common name/Synonyms ZKO
Strain types mutant
Background strain 129
Institution Clinical Laboratory Science / Biological Chemistry, Graduate School of Medicine, Kyoto University
Developer Hiromu Takematsu
Depositor Hiromu Takematsu
Conditions of distribution

In publishing the research results obtained by use of the animal resource, a citation of the following literature (ref.1) and the stipulation of an acknowledgment to depositor designated by the depositor is requested. It's necessary to obtain depositor's consent on use of biological resources. Contact us

Animal Health Report
Strain description

The Cmah-KO(129) mice are knockout mice deficient for the enzyme cytidine monophospho-N-acetylneuraminic acid hydroxylase (Cmah), which catalyzes the converting reaction from N-acetylneuraminic acid to N-glycolylneuraminic acid in sialylated molecules. Therefore, the mice have sialylated molecules with N-acetylneuraminic acid only. Systemic effect of the Cmah deficiency is not entirely revealed yet, but the Cmah-KO mice showed hyperresponsive B cell phenotypes in assays measuring B-cell functions, i.e., antibody production and proliferation. The mice also showed activation-dependent dynamic reduction of CD22 ligand expression, and great increase of ligand expression for sialoadhesin (Siglec-1, CD169).

Humans (Homo sapiens) lack the Cmah gene unlike chimpanzee and bonobo, in which the Cmah gene is conserved. The Cmah-KO mice can be used as models for humans in terms of the sialic acid species in sialylated molecules. In this sense, the Cmah-KO strain serves as a base strain for creating mouse models for medical research in human diseases.

Care should be taken for the proliferation of the mice because their reproductive performances are not good.

References
  1. Naito Y, Takematsu H, Koyama S, Miyake S, Yamamoto H, Fujinawa R, Sugai M, Okuno Y, Tsujimoto G, Yamaji T, Hashimoto Y, Itohara S, Kawasaki T, Suzuki A, Kozutsumi Y. Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B-cell activation. Mol Cell Biol. 2007 Apr;27(8):3008-22. Epub 2007 Feb 12. PMID: 17296732
Resource information
Cryopreserved sperm In-house Genotype Hemozygous
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System IVF
Genotype heterozygous and homozygous
Medium EFS40
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types knockout
Gene symbol Cmah
Gene name cytidine monophospho-N-acetylneuraminic acid hydroxylase
Genotyping information