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Strain Detail

Strain information
Resource No. nbio020
Strain name GFP
Strain Nomenclature C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb
Common name/Synonyms Green Mouse
Strain types mutant
Background strain C57BL/6N
Institution Genome Infomation Research Center with Research Institute for Microbial Diseases, Osaka University
Developer Masaru Okabe
Depositor Masaru Okabe
Conditions of distribution Please contact to the depositor (HP) and execute a MTA with.
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Animal Health Report
Strain description

GFP mice is transgenic mice expressing Green Fluorescent Protein gene (act-EGFP) derived from the jellyfish Aequorea victoria in whole body. Background strain is C57BL/6N. We cryopreserve heterozygous embryos now. Cells expressing GFP are available for cell transplant experiments or organ transplant experiments to be able to visualize targets by radiating UV or blue light.

Photo of GFP mice

GFP mice (3 mice on the right are radiating fluorescent green under UV light.)

References
  1. Ikawa M, Yamada S, Nakanishi T, Okabe M. Green fluorescent protein (GFP) as a vital marker in mammals. Curr Top Dev Biol. 1999; 44:1-20.
  2. Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y. 'Green mice' as a source of ubiquitous green cells. FEBS Lett. 1997 May 5; 407(3): 313-9.
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol Gfp (EGFP)
Gene name Green fluorescent protein (Enhanced Green Fluorescent Protein)
Genotyping information