Strain Detail: GLUT4-NF1/A mice

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GLUT4-NF1/A mice express transgene included exon and intron of mouse GLUT4 inserted CAT tag in the 3′-untranslated region and 3,000 bp of 5′-upstream region introduced point mutation in the NF1 site. This strain is available for analysis of GLUT4 promoter. This strain has 4 lines, A, B, D-L, D-H. GLUT4, one of the glucose transporter which is rate-limiting step of importing sugars in muscle or fat tissue, plays an important role of blood sugar homeostasis. This strain is available for diabetes-related research because increasing/decreasing GLUT4 expression level is related to mechanism of onset or preventation of diabetes.

1. Ikemoto S, Thompson KS, Itakura H, Lane MD, and Ezaki O. Expression of an insulin-responsive glucose transporter (GLUT4) minigene in transgenic mice: Effect of exercise and role in glucose homeostasis.Proc. Natl. Acad. Sci. USA, 92, 865-869 (1995). [PMID: 7846068]
2. Tsunoda N, Cooke DW, Ikemoto S, Maruyama K, Takahashi M, Lane MD, and Ezaki O. Regulated expression of 5′-deleted mouse GLUT4 minigenes in transgenic mice: effects of exercise training and high-fat diet.Biochem. Biophys. Res. Commun. 239, 503-509 (1997). [PMID: 9344860]
3. Miura, S., Tsunoda, N., Ikeda, S., Kai, Y., Ono, M., Maruyama, K., Takahashi, M. Mochida, K., Matsuda, J., Lane, M.D., and Ezaki, O. Regulatory sequence elements of mouse GLUT4 gene expression in adipose tissues.Biochem. Biophys. Res. Commun. 312, 277-284 (2003). [PMID: 14637133]
4. Miura, S., Tsunoda, N., Ikeda, S., Kai, Y., Cooke, D.W., Lane, M.D., and Ezaki, O. Nuclear factor 1 regulates adipose tissues-specific expression in the mouse GLUT4 gene.Biochem. Biophys. Res. Commun. 325, 812-818 (2004).[PMID: 15541363]