HOME > mice > Gnat1-/- RPE65Leu450 mice
Strain Detail: Gnat1-/- RPE65Leu450 mice
| Strain information |
| Resource No. |
nbio421 |
| Strain name |
Gnat1-/- RPE65Leu450 |
| Strain Nomenclature |
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| Common name/Synonyms |
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| Strain types |
mutant |
| Background strain |
C57BL/6J |
| Institution |
National Cerebral and Cardiovascular Center (JAPAN) |
| Developer |
Shinya Sato |
| Depositor |
Shinya Sato |
| Conditions of distribution |
without conditions: contact us |
| Animal Health Report |
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| Strain description |
Mice deficient in the rod transducin α subunit (Gnat1). The mice are useful for analysis of the response of cone photoreceptor cells, as rod photoreceptor cells of the mice do not respond to light. In addition, the 450th residue of RPE65 in the normal C57BL/6 is mutated to methionine, resulting in reduced RPE65 enzyme activity, but in this strain, the residue is returned to leucine (RPE65Leu450) to restore activity. RPE65Leu450 was derived from the 129 line ES cells (W9.5 cell line) used to create knockout mice. The RPE65Leu450 allele was left behind in the process of creating C57BL/6 congenic mice from mice derived from 129 ES cells (W9.5 cell line) containing Gnat1 knockout alleles. As a result, the increase speed in visual sensitivity in the dark is restored. |
| Reference(s) |
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| Resource information |
| Cryopreserved sperm |
In-house |
Genotype |
Gnat1: homozygous, RPE65Leu450: heterozygous |
| Medium |
Fertiup |
| From other institutions |
Genotype |
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| Medium |
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| Cryopreserved embryo |
In-house |
Mating System |
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| Genotype |
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| Medium |
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| From other institutions |
Mating System |
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| Genotype |
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| Method |
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| Strain status / Availability |
Cryopreserved sperm |
Within 1 month |
| Cryopreserved embryo |
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| Live animals |
Approx. 2 months |
| Gene information |
| Mutation types |
knockout/mutant |
| Gene symbol |
Gnat1, Rpe65 |
| Gene name |
G protein subunit alpha transducin 1, retinal pigment epithelium 65 |
| Genotyping information |
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