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Strain Detail: hyBRET-ERK-NES mice

Strain information
Resource No. nbio325
Strain name hyBRET-ERK-NES
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. contact us
Animal Health Report
Strain description

Genetically encoded biosensors based on the principle of Förster resonance energy transfer comprise two classes: biosensors based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET). Various FRET biosensors have been developed as tools to visualize signaling-molecule activity in live cells or live animals. However, these FRET biosensors are challenging to be applied to a drug screening system targeting intracellular signal transduction molecules because many low molecular weight compounds for drug screening have fluorescence and the molecular activity cannot be monitored due to the competition of their fluorescence with that of the FRET biosensor. BRET biosensors are suitable for drug screening because of no such drawbacks, but their emission is not bright enough for in vivo bioimaging. To solve this problem, Dr. Matuda‚Äôs group developed BRET-FRET hybrid biosensors called hyBRET biosensors. These hyBRET biosensors can be used as FRET biosensors with blue light, and as BRET biosensors with coelenterazine (luciferase substrates).

Mice of this strain are transgenic mice expressing cytoplasm-localized hyBRET biosensors monitoring ERK kinase activity under the control of CAG promoter in their entire body.

  1. Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada Y, Nagai T, and Matsuda M. (2018) A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging. Sci Rep 8(1):8984. [PMID:29895862]
Resource information
Cryopreserved sperm In-house Genotype Tg/+
Medium Fertiup
From other institutions Genotype
Cryopreserved embryo In-house Mating System IVF with Tg/+ sperm and oocytes fromB6N-Tyrc-Brd/BrdCrCrl (B6-Albino)
Genotype Mix of heterozygotes and wild-types
Medium EFS40
From other institutions Mating System
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol CFP, YFP, Rluc
Gene name cyan fluorescent protein, yellow fluorescent protein, Renilla Luciferase
Genotyping information