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Strain Detail

Strain information
Resource No. nbio146
Strain name L1-EGFP#57
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain
Institution National Center for Global Health and Medicine
Developer
Depositor Tadashi Okamura
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

L1-EGFP#57 mice are transgenic mice to be able to monitor LINE1 dynamics by using EGFP expression as a indicator. This strain is available for assay system of carcinogen. These mice express ORF1 and ORF2 which are human L1 elements under the control of their own promoter, and express EGFP when transgene is introduced in genome by retro transposition. This strain has 16 lines. It's possible to supply mL1-EGFP mice which express mouse L1 elements.

References
  1. Okudaira N, Goto M, Yanobu-Takanashi R, Tamura M, An A, Abe Y, Kano S, Hagiwara S, Ishizaka Y, Okamura T. Involvement of retrotransposition of long interspersed nucleotide element-1 in skin tumorigenesis induced by 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate. Cancer Sci. 2011 Nov;102(11):2000-6. doi: 10.1111/j.1349-7006.2011.02060.x. Epub 2011 Sep 19.
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol LINE1, EGFP
Gene name Long interspersed nuclear element 1, enhanced green fluorescent protein
Genotyping information Genotyping PCR protocol for L1-EGFP mice (pdf).