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Strain Detail

Strain information
Resource No. nbio208
Strain name lox-PKAchu
Strain Nomenclature B6C3F1-Tg(pT2A-lox-tdKeima-3901NES)#1
Common name/Synonyms
Strain types mutant
Background strain
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

The lox-PKAchu mice are transgenic mice expressing the probe which is consisted of cytoplasm-localized förster (or fluorescence) resonance energy transfer (FRET) biosensor monitoring PKA kinase activity added with lox-tdKeima. Protein kinase A (PKA, also known as the cyclic AMP-dependent protein kinase) is activated by cAMP which is raised intracellular concentration by stimuli of hormone or bioactive substance, and relays signal transduction received like hormones. This strain expresses Keima fluorescent protein under the control of CAG promoter in almost all tissues. These mice are able to switch expression tissue-specifically from Keima fluorescent protein to FRET biosensor by mating with tissue-specific Cre-expressed mouse. It's possible to measure PKA kinase activity spatially and temporally in fetal or adult various tissues by live imaging observation of this strain in the two-photon microscopy. This strain expects not only to analyze the signal transduction cascade in live animals, but also apply drug development such as the pharmacodynamic analysis targeting signal molecules, because these mice are showed rich expression in primary cultured cells derived from various tissues. In the developmental stage, biosensor expression can be observed at almost all cells. FRET biosensor generally can't use for fixed samples.

References
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol CFP, YFP, tdKeima
Gene name Cyan Fluorescent Protein, Yellow Fluorescent Protein, tandem dimer of Keima
Genotyping information