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Strain Detail

Strain information
Resource No. nbio116
Strain name Meg1 Tg/T20L
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain
Institution Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University1, Tokai University School of Health Sciences2
Developer Fumitoshi Ishino1, Tomoko Kaneko-Ishino2
Depositor Fumitoshi Ishino1, Tomoko Kaneko-Ishino2
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

Meg1 Tg/T20L mice are transgenic mice overexpressing mouse Meg1/Grb10 in whole body. Meg1/Grb10 is an imprinting gene to express from only maternal genome. CAG promoter is used for transgene expression. This strain shows poor growth after weaning and insulin resistance, then onset of diabetes. Furthermore, this strain increases diabetes incidence rate by high nutritional feed. This strain is type 2 diabetes model mice. These mice become polyuria with onset of diabetes, therefore it's necessary to change mouse bedding frequently. This strain has different 4 lines from expression level.

References
  1. Shiura H, Miyoshi N, Konishi A, Wakisaka-Saito N, Suzuki R, Muguruma K, Kohda T, Wakana S, Yokoyama M, Ishino F, Kaneko-Ishino T. Meg1/Grb10 overexpression causes postnatal growth retardation and insulin resistance via negative modulation of the IGF1R and IR cascades. Biochem Biophys Res Commun. 15;329(3):909-16, 2005. [PMID: 15752742]
  2. Yamamoto Y, Ishino F, Kaneko-Ishino T, Shiura H, Uchio-Yamada K, Matsuda J, Suzuki O, and Sato K. Type 2 Diabetes Mellitus in a Non-Obese Mouse Model Induced by Meg1/Grb10 Overexpression. Exp Anim 57(4):385-395, 2008. [PMID: 18633161]
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol Meg1 (Grb10)
Gene name Meiosis expressed gene 1 (Growth factor receptor-bound protein 10)
Genotyping information