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Strain Detail

Strain information
Resource No. nbio190
Strain name Pippi
Strain Nomenclature (ICR×B6C3F1)F1-Tg(pT2A-1759xRe)
Common name/Synonyms Pippi-PI3,4P2 mice
Strain types mutant
Background strain
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

Pippi mice are transgenic mice expressing förster (or fluorescence) resonance energy transfer (FRET) biosensor Pippi-PI3,4P2 monitoring PI3,4P2 level on the plasma membrane under the control of CAG promoter in whole body. PI3,4P2 is produced when PIP3 produced by PI3 kinases is dephosphorylated, and activates lots of enzymes included PH domain. Especially, because it's known that PDK and Akt which are kinases regulating apoptosis and cell movement bind to both PIP3 and PI3,4P2, PI3,4P2 is often used as indicator of activation level for these molecle. It's possible to measure PI3,4P2 level spatially and temporally in fetal or adult various tissues by live imaging observation of this strain in the two-photon microscopy. This strain expects not only to analyze the signal transduction cascade in live animals, but also apply drug development such as the pharmacodynamic analysis targeting PI3K, because these mice are showed rich expression in primary cultured cells derived from various tissues. This biosensor expresses in plasma membrane. FRET biosensor generally can't use for fixed samples.

References
  1. Kamioka Y, Sumiyama K, Mizuno R, Sakai Y, Hirata E, Kiyokawa E, Matsuda M. Live Imaging of Protein Kinase Activities in Transgenic Mice Expressing FRET Biosensors. Cell Struct Funct. 2012 Jan 24. [Epub ahead of print] PMID: 22277578
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol CFP, YFP, Cdc42
Gene name Cyan Fluorescent Protein, Yellow Fluorescent Protein, Cell division cycle 42
Genotyping information