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Strain Detail: PKAchu Gnat1-/- mice

Strain information
Resource No. nbio373
Strain name PKAchu Gnat1-/-
Strain Nomenclature
Common name/Synonyms B6-Tg(AKAR3EV)pT2A-3901NES Gnat1-/-
Strain types mutant
Background strain C57BL/6
Institution Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. contact us
Animal Health Report
Strain description

Transgenic mice systemically expressing the FRET biosensor AKAR3EV (cytoplasmic localized), which monitors kinase activity of PKA, under the CAG promoter. PKAchu (nbio185) was crossed with Gnat1-/- mice lacking rod transducin α subunit and mice. Retinal PKA activity imaging can be performed under conditions that no visual light response occurs in rod photoreceptor cells.

  1. Kamioka Y, Sumiyama K, Mizuno R, Sakai Y, Hirata E, Kiyokawa E, and Matsuda M. (2012) Live imaging of protein kinase activities in transgenic mice expressing FRET biosensors. Cell Struct Funct 37(1):65-73. [PMID:22277578]
  2. Calvert PD, Krasnoperova NV, Lyubarsky AL, Isayama T, Nicoló M, Kosaras B, Wong G, Gannon KS, Margolskee RF, Sidman RL, Pugh EN, Makino CL, and Lem J. (2000) Phototransduction in transgenic mice after targeted deletion of the rod transducin alpha -subunit. Proc Natl Acad Sci U S A 97(25):13913-8. [PMID:11095744]
  3. Sato S, Yamashita T, and Matsuda M. (2020) Rhodopsin-mediated light-off-induced protein kinase A activation in mouse rod photoreceptor cells. Proc Natl Acad Sci U S A 117(43):26996-27003. [PMID:33046651]
Resource information
Cryopreserved sperm In-house Genotype
From other institutions Genotype
Cryopreserved embryo In-house Mating System
From other institutions Mating System
Strain status / Availability Cryopreserved sperm
Cryopreserved embryo
Live animals Only live mice are available (Approx. 2 months).
Gene information
Mutation types transgenic
Gene symbol CFP, YFP, Gnat1
Gene name Cyan Fluorescent Protein, Yellow Fluorescent Protein, G protein subunit α transducin 1
Genotyping information
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