HOME > mice > PKAchu Gnat1-/- mice
Strain Detail: PKAchu Gnat1-/- mice
| Strain information |
| Resource No. |
nbio373 |
| Strain name |
PKAchu Gnat1-/- |
| Strain Nomenclature |
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| Common name/Synonyms |
B6-Tg(AKAR3EV)pT2A-3901NES Gnat1-/- |
| Strain types |
mutant |
| Background strain |
C57BL/6 |
| Institution |
Graduate School of Biostudies, Kyoto University |
| Developer |
Michiyuki Matsuda |
| Depositor |
Michiyuki Matsuda |
| Conditions of distribution |
It's necessary to obtain depositor's consent on use of biological resources. contact us |
| Animal Health Report |
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| Strain description |
Transgenic mice systemically expressing the FRET biosensor AKAR3EV (cytoplasmic localized), which monitors kinase activity of PKA, under the CAG promoter. PKAchu (nbio185) was crossed with Gnat1-/- mice lacking rod transducin α subunit and mice. Retinal PKA activity imaging can be performed under conditions that no visual light response occurs in rod photoreceptor cells. |
| Reference(s) |
- Kamioka Y, Sumiyama K, Mizuno R, Sakai Y, Hirata E, Kiyokawa E, and Matsuda M. (2012) Live imaging of protein kinase activities in transgenic mice expressing FRET biosensors. Cell Struct Funct 37(1):65-73. [PMID:22277578]
- Calvert PD, Krasnoperova NV, Lyubarsky AL, Isayama T, Nicoló M, Kosaras B, Wong G, Gannon KS, Margolskee RF, Sidman RL, Pugh EN, Makino CL, and Lem J. (2000) Phototransduction in transgenic mice after targeted deletion of the rod transducin alpha -subunit. Proc Natl Acad Sci U S A 97(25):13913-8. [PMID:11095744]
- Sato S, Yamashita T, and Matsuda M. (2020) Rhodopsin-mediated light-off-induced protein kinase A activation in mouse rod photoreceptor cells. Proc Natl Acad Sci U S A 117(43):26996-27003. [PMID:33046651]
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| Resource information |
| Cryopreserved sperm |
In-house |
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| From other institutions |
Genotype |
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| Cryopreserved embryo |
In-house |
Mating System |
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| From other institutions |
Mating System |
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| Strain status / Availability |
Cryopreserved sperm |
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| Cryopreserved embryo |
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| Live animals |
Only live mice are available (Approx. 2 months). |
| Gene information |
| Mutation types |
transgenic |
| Gene symbol |
CFP, YFP, Gnat1 |
| Gene name |
Cyan Fluorescent Protein, Yellow Fluorescent Protein, G protein subunit α transducin 1 |
| Genotyping information |
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