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Strain Detail: Raichu-Cdc42-F0 mice

Strain information
Resource No. nbio191
Strain name Raichu-Cdc42-F0
Strain Nomenclature B6C3F1-Tg(pT2A-Raichu-2253x)
Common name/Synonyms Cdc42-F0 mice
Strain types mutant
Background strain
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. contact us
Animal Health Report
Strain description

Raichu-Cdc42-F0 mice are transgenic mice expressing förster (or fluorescence) resonance energy transfer (FRET) biosensor Raichu-Cdc42 under the control of CAG promoter in whole body. Cdc42 is a member of Rho family small GTP biding protein. It is activated in filopodia and regulates cell movement through the induction of actin cytoskeleton polymerization. It's possible to measure Cdc42 activity spatially and temporally in fetal or adult various tissues by live imaging observation of this strain in the two-photon microscopy. This strain expects not only to analyze the signal transduction cascade in live animals, but also apply drug development such as the pharmacodynamic analysis targeting Cdc42, because these mice are showed rich expression in primary cultured cells derived from various tissues. This biosensor expresses in plasma membrane. FRET biosensor generally can't use for fixed samples.

  1. Kamioka Y, Sumiyama K, Mizuno R, Sakai Y, Hirata E, Kiyokawa E, and Matsuda M. (2012) Live imaging of protein kinase activities in transgenic mice expressing FRET biosensors. Cell Struct Funct 37(1):65-73. [PMID:22277578]
Resource information
Cryopreserved sperm In-house Genotype
From other institutions Genotype
Cryopreserved embryo In-house Mating System
Medium EFS40
From other institutions Mating System
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol CFP, YFP, Cdc42
Gene name Cyan Fluorescent Protein, Yellow Fluorescent Protein, Cell division cycle 42
Genotyping information