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Strain Detail: Tak1flox mice

Strain information
Resource No. nbio060
Strain name Tak1flox
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain
Institution Research Institute for Microbial Diseases, Osaka University
Developer Shizuo Akira
Depositor Shizuo Akira
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. contact us
Animal Health Report
Strain description

TGF-β-activated kinase 1 (TAK1) is belong to mammalian MAP kinase kinase kinase (MAPKKK) family encoding in Map3k7 gene.

TAK1flox mice is established by crossing between C57BL/6 strain and chimera by using ES cells derived from 129/Ola strain (E14.1) introduced two loxP sites flanking exon 2 of Map3k7 by gene targeting. It's possible to delete TAK1 gene by crossing with Cre expressing mice. Especially, it's possible of stage- or organ-specific delation by selecting promoter induced expression of Cre recombinase, thus TAK1flox mice are useful for the research of stage- or tissue-specific TAK1.

Though TAK1 knockout mice were also produced by crossing with mice expressing Cre in germ cells, these mice were embryonic lethal (1). Furthermore, Tak1flox mice are observed abnormality of Toll-like receptor/IL-1R signal by tissue-specific knockout.
Reference(s)
  1. Sato S, Sanjo H, Takeda K, Ninomiya-Tsuji J, Yamamoto M, Kawai T, Matsumoto K, Takeuchi O, and Akira S. (2005) Essential function for the kinase TAK1 in innate and adaptive immune responses. Nat. Immunol. 6(11):1087-95. [PMID:16186825]
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types conditional KO
Gene symbol Tak1, Map3k7
Gene name TGF-β-activated kinase 1, Mitogen-activated protein kinase kinase kinase 7
Genotyping information