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Strain Detail: Ubc13flox mice
|Institution||Research Institute for Microbial Diseases, Osaka University|
|Conditions of distribution||It's necessary to obtain depositor's consent on use of biological resources. contact us|
|Animal Health Report|
Ubc13, an enzyme classified as ubiquitin conjugating enzyme (E2), constructs poly-ubiquitin chains to add ubiquitin to lysine 63 (K63). K63-linked poly-ubiquitin chains is related to signal transduction and DNA repair to function as protein binding domains. For example, the addition of K63-linked poly-ubiquitin chains to target proteins by Ubc13 is essential for signal transduction of immunoreceptor induced to activate transcription factor NF-κB. Akiraet al. showed that Ubc13 palyed an important role of macrophage activation and B-cell formation or function to examine an effect of tissue-specific inactivation of Ubc13 gene by using Ubc13flox mice (1).
This strain is established by crossing between C57BL/6 strain and chimera by using ES cells derived from 129/Ola strain (E14.1) introduced two loxP sites flanking exon 2, 3 and 4 of Ubc13 gene by gene targeting. It's possible to inactivate Ubc13 gene by crossing with Cre expressing mice. It's possible to inactivate NDRG1 stage- or tissue-specifically by selecting different promoter inducing to express Cre recombinase, thus this strain is available for research of stage- or tissue-specific Ubc13.
On the one hand, "Ubc13 knockout mice" ware also produced, but embryonic lethal (1). Furthermore, Ubc13flox mice are observed abnormality of Toll-like receptor/IL-1R signal by tissue-specific knockout.
|From other institutions||Genotype|
|Cryopreserved embryo||In-house||Mating System|
|From other institutions||Mating System|
|Strain status / Availability||Cryopreserved sperm||Within 1 month|
|Cryopreserved embryo||Within 1 month|
|Live animals||Approx. 2 months|
|Mutation types||conditional KO|
|Gene name||Ubiquitin carrier protein 13|