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Strain Detail

Strain information
Resource No. nbio067
Strain name Ubc13flox
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain
Institution Research Institute for Microbial Diseases, Osaka University
Developer Shizuo Akira
Depositor Shizuo Akira
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

Ubc13, an enzyme classified as ubiquitin conjugating enzyme (E2), constructs poly-ubiquitin chains to add ubiquitin to lysine 63 (K63). K63-linked poly-ubiquitin chains is related to signal transduction and DNA repair to function as protein binding domains. For example, the addition of K63-linked poly-ubiquitin chains to target proteins by Ubc13 is essential for signal transduction of immunoreceptor induced to activate transcription factor NF-κB. Akira et al. showed that Ubc13 palyed an important role of macrophage activation and B-cell formation or function to examine an effect of tissue-specific inactivation of Ubc13 gene by using Ubc13flox mice (1).

This strain is established by crossing between C57BL/6 strain and chimera by using ES cells derived from 129/Ola strain (E14.1) introduced two loxP sites flanking exon 2, 3 and 4 of Ubc13 gene by gene targeting. It's possible to inactivate Ubc13 gene by crossing with Cre expressing mice. It's possible to inactivate NDRG1 stage- or tissue-specifically by selecting different promoter inducing to express Cre recombinase, thus this strain is available for research of stage- or tissue-specific Ubc13.

On the one hand, "Ubc13 knockout mice" ware also produced, but embryonic lethal (1). Furthermore, Ubc13flox mice are observed abnormality of Toll-like receptor/IL-1R signal by tissue-specific knockout.

References
  1. Yamamoto M, Okamoto T, Takeda K, Sato S, Sanjo H, Uematsu S, Saitoh T, Yamamoto N, Sakurai H, Ishii KJ, Yamaoka S, Kawai T, Matsuura Y, Takeuchi O, Akira S. Key function for the Ubc13 E2 ubiquitin-conjugating enzyme in immune receptor signaling. Nat Immunol 2006;7: 962-970.
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types conditional KO
Gene symbol Ubc13
Gene name Ubiquitin carrier protein 13
Genotyping information