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Strain Detail: B3gat1 KO

Strain information
Resource No. nbio263
Strain name B3gat1 KO
Strain Nomenclature
Common name/Synonyms GlcAT-P
Strain types mutant
Background strain C57BL/6J (~N30)
Institution Clinical Laboratory Science / Biological Chemistry, Graduate School of Medicine, Kyoto University
Developer Shogo Oka
Depositor Shogo Oka
Conditions of distribution

In publishing the research results obtained by use of the animal resource, a citation of the following literature (ref.1) and the stipulation of an acknowledgment to depositor designated by the depositor is requested. It's necessary to obtain depositor's consent on use of biological resources. Contact us

Animal Health Report
Strain description

The B3gat1 KO mice are knockout mice deficient for galactosylgalactosylxylosylprotein 3-β-glucuronosyltransferase 1 (B3Gat1, aka., glucuronosyltransferase P, GlcAT-P), a synthetic enzyme of a neural specific carbohydrate antigen, the human natural killer-1 (HNK-1) carbohydrate antigen. The HNK-1 carbohydrate antigen is synthesized by a transfer of glucuronic acid to N-acetyllactosamine at non-reduced termini of carbohydrates by glucuronyltransferases: GlcAT-P (B3gat1) or GlcAT-S (B3gat2) and then a transfer of sulfate groups by natural killer-1 sulfotransferase (HNK-1ST). In the brain, GlcAT-P (B3gat1) is a major glucuronyltransferase involved in the HNK-1 carbohydrate antigen synthesis. The B3gat1 KO mice exhibited normal development of gross anatomical features, but the adult mutant mice exhibited reduced long-term potentiation at the Schaffer collateral-CA1 synapses and a defect in spatial memory formation. The strain is useful as a mouse model for analyzing high-order functions of the brain.

References
  1. Yamamoto S, Oka S, Inoue M, Shimuta M, Manabe T, Takahashi H, Miyamoto M, Asano M, Sakagami J, Sudo K, Iwakura Y, Ono K, Kawasaki T. Mice deficient in nervous system-specific carbohydrate epitope HNK-1 exhibit impaired synaptic plasticity and spatial learning. J Biol Chem. 2002 Jul 26;277(30):27227-31. PMID: 12032138
Resource information
Cryopreserved sperm In-house Genotype Heterozygous
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System IVF
Genotype mix of KO/+ and wild-type
Medium EFS40
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types knockout
Gene symbol B3gat1
Gene name β-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Genotyping information