Strain Detail: B3gat1 KO mice

In publishing the research results obtained by use of the animal resource, a citation of the following literature (ref.1) and the stipulation of an acknowledgment to depositor designated by the depositor is requested. It's necessary to obtain depositor's consent on use of biological resources.Contact us

The B3gat1 KO mice are knockout mice deficient for galactosylgalactosylxylosylprotein 3-β-glucuronosyltransferase 1 (B3Gat1, aka., glucuronosyltransferase P, GlcAT-P), a synthetic enzyme of a neural specific carbohydrate antigen, the human natural killer-1 (HNK-1) carbohydrate antigen. The HNK-1 carbohydrate antigen is synthesized by a transfer of glucuronic acid to N-acetyllactosamine at non-reduced termini of carbohydrates by glucuronyltransferases: GlcAT-P (B3gat1) or GlcAT-S (B3gat2) and then a transfer of sulfate groups by natural killer-1 sulfotransferase (HNK-1ST). In the brain, GlcAT-P (B3gat1) is a major glucuronyltransferase involved in the HNK-1 carbohydrate antigen synthesis. The B3gat1 KO mice exhibited normal development of gross anatomical features, but the adult mutant mice exhibited reduced long-term potentiation at the Schaffer collateral-CA1 synapses and a defect in spatial memory formation. The strain is useful as a mouse model for analyzing high-order functions of the brain.

1. Yamamoto S, Oka S, Inoue M, Shimuta M, Manabe T, Takahashi H, Miyamoto M, Asano M, Sakagami J, Sudo K, Iwakura Y, Ono K, and Kawasaki T. (2002) Mice deficient in nervous system-specific carbohydrate epitope HNK-1 exhibit impaired synaptic plasticity and spatial learning. J Biol Chem 277(30):27227-31. [PMID:12032138]