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Strain Detail: B3gat2 KO mice

Strain information
Resource No. nbio372
Strain name B3gat2 KO
Strain Nomenclature
Common name/Synonyms GlcAT-S KO
Strain types mutant
Background strain
Institution Department of Biological Chemistry, Human Health Sciences, Graduate School of Medicine, Kyoto University
Developer Shogo Oka
Depositor Shogo Oka
Conditions of distribution Users shall cite references specified by the depositor when publishing research results. Users shall give an acknowledgment to the depositor when publishing the research results. Users shall obtain the consent of the depositor in advance. contact us
Animal Health Report
Strain description

This B3gat2 KO strain is a mouse strain lacking the B3gat2 (GlcAT-S) enzyme. B3gat2 (GlcAT-S) is an enzyme highly expressed in the nervous system and is responsible for the biosynthesis of an HNK-1 carbohydrate antigen. The HNK-1 carbohydrate antigen is biosynthesized by transferring glucuronic acid by GlcAT-P (B3gat1) or GlcAT-S (B3gat2) to the N-acetyllactosamine structure at the non-reducing end of the carbohydrate chain and then transferring the sulfate group by HNK-1ST. Since GlcAT-P (B3gat1) is the major glucuronosyltransferase involved in the synthesis of HNK-1 glycans in the brain, genetic deletion of GlcAT-S (B3gat2) causes little change in the expression of HNK-1 glycans in the brain. On the other hand, since GlcAT-S (B3gat2) is highly expressed in the kidney, deletion of this gene abolishes the expression of non-sulfated HNK-1 carbohydrate chains in the kidney (HNK-1ST is not expressed in the kidney and therefore does not undergo sulfation). It is known that the expression of non-sulfated HNK-1 sugar chains in the kidney increases with aging in wild-type mice. Since LacZ is incorporated in the knockout allele, the expression site of GlcAT-S (B3gat2) can be visualized using Xgal staining.

  1. Yabuno K, Morise J, Kizuka Y, Hashii N, Kawasaki N, Takahashi S, Miyata S, Izumikawa T, Kitagawa H, Takematsu H, Oka S. (2015) A Sulfated Glycosaminoglycan Linkage Region is a Novel Type of Human Natural Killer-1 (HNK-1) Epitope Expressed on Aggrecan in Perineuronal Nets. PLoS One. 10:e0144560. [PMID: 26659409]
Resource information
Cryopreserved sperm In-house Genotype heterozygous
From other institutions Genotype
Cryopreserved embryo In-house Mating System
Genotype heterozygous/wild-type
From other institutions Mating System
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types knockout
Gene symbol B3gat2 (GlcAT-S)
Gene name β-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)
Genotyping information