HOME > mice > CD40L Tg mice

Strain Detail

Strain information
Resource No. nbio010
Strain name CD40L Tg
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain
Institution Tokyo Medical and Dental University
Developer Takeshi Tsubata
Depositor
Conditions of distribution Only for collaboration with the depositor : contact us
Animal Health Report
Strain description

CD40L transgenic mice were established by injecting the DNA fragment containing the mouse CD40L cDNA, a VH promoter, the IgH intron enhancer, and the Ig?3' enhancer into C57BL/6 fertilized eggs. This strain ectopically expresses CD40 ligands on B cells with the C57BL/6 background. This strain is a good model for systemic lupus erythematosus (SLE).

Some of the 12- to 14-mo-old CD40L-transgenic mice spontaneously produced autoantibodies, such as anti-DNA antibodies, and anti-histone antibodies. Approximately half of the transgenic mice developed glomerulonephritis with immune-complex deposition.

What are CD40 and CD40L?

CD40 is a member of TNFR family and is expressed in cells such as B cells, macrophages, and dendritic cells. CD40 ligand (CD40L) is a member of the TNF ligand family, expressed mainly on activated T cells. CD40/CD40L play an important role in cell activation. In B cells, interation with CD40L promotes proliferation and survival of B cells, Ig isotype switching.

What is Systemic Lupus Erythematosus (SLE)?

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease manifesting inflammatory damage in a variety of organs including glomerulonephritis, myocarditis and pericarditis. SLE is characterized by production of autoantibodies to nuclear components such as DNA, histone. The etiology of SLE involves both genetic and environmental factors, although the details are largely unknown.

References
  1. Higuchi T, Aiba Y, Nomura T, Matsuda J, Mochida K, Suzuki M, Kikutani H, Honjo T, Nishioka K, Tsubata T. Cutting Edge: Ectopic expression of CD40 ligand on B cells induces lupus-like autoimmune disease. J Immunol, 168:9-12, 2002.
Resource information
Cryopreserved sperm In-house Genotype Tg/+
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype Tg/+
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol Cd40lg
Gene name CD40 ligand
Genotyping information