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Strain Detail: C57BL/6-Tg(EGFP/E8)Kyo mice

Strain information
Resource No. nbio224
Strain name C57BL/6-Tg(EGFP/E8)Kyo
Strain Nomenclature C57BL/6-Tg(EGFP/E8)Kyo
Common name/Synonyms EGFP/E8 Tg
Strain types mutant
Background strain
Institution Department of Animal Development and Physiology, Kyoto University
Developer
Depositor Kazuhiro Sakamaki
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. contact us
Animal Health Report
Strain description

EGFP/E8 Tg mice are transgenic mice which produce E8 protein by the recombination of Equine herpesvirus 2 E8 gene from EGFP in the presence of Cre recombinase. These mice are possible to detect existence of E8 protein by using Flag antibody because of the addition of Flag tag in the amino terminal of E8 protein (ref.1). These mice are also able to detect GFP in the skin under UV light. Equine herpesvirus 2 E8 protein is also known as FLIP (FLICE/caspase-8-Inhibitory Protein), apoptosis inhibitory protein, thus this strain is used for the research of apoptosis.
Reference(s)
  1. OhYama T, Tsukumo S, Yajima N, Sakamaki K, and Yonehara S. (2000) Reduction of thymocyte numbers in transgenic mice expressing viral FLICE-inhibitory protein in a Fas-independent manner. Microbiol Immunol 44(4):289-97. [PMID:10832975]
Resource information
Cryopreserved sperm In-house Genotype
Medium
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol EGFP, EhV2gp75 ORF E8
Gene name Enhanced Green Fluorescent Protein, EhV2gp75
Genotyping information