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Strain Detail: Far2-KO mice

Strain information
Resource No. nbio462
Strain name Far2-KO
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain C57BL/6J
Institution Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University
Developer Akio Kihara
Depositor Akio Kihara
Conditions of distribution Users shall give an acknowledgment to the depositors when publishing the research results. contact us
Animal Health Report
Strain description
Faces of Far2-KO and normal miceEyelids of Far2-KO and normal mice

Far2 is an isozyme of fatty acyl-CoA reductase (FAR), which synthesizes aliphatic alcohols from acyl-CoA. Far2 is highly expressed in the meibomian glands and is involved in the production of wax esters, the major lipid component of the tear film. Far2 knockout mice exhibit dry eye symptoms such as meibomian gland blockage, tear film instability and corneal damage.
Reference(s)
  1. Otsuka K, Sawai-Ogawa M, and Kihara A. (2022) Formation of fatty alcohols-components of meibum lipids-by the fatty acyl-CoA reductase FAR2 is essential for dry eye prevention. FASEB J 36(4):e22216. [PMID:35238077]
Resource information
Cryopreserved sperm In-house Genotype Heterozygous
Medium Fertiup
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System ♂: Heterozygous x ♀: B6J wild-type
Genotype Heterozygous or wild-type
Medium EFS40
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm
Cryopreserved embryo
Live animals
Gene information
Mutation types knockout
Gene symbol Far2
Gene name fatty acyl CoA reductase 2
Genotyping information

The genotypes are determined by the band pattern after Sau3AI digestion of the PCR products (see far2-ko-genotyping.pdf).