HOME > mice > Hps5-KO mice

Strain Detail: Hps5-KO mice

Strain information
Resource No. nbio337
Strain name Hps5-KO
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain C57BL/6N
Institution Laboratory of Animal Models for Human Diseases, National Institutes of Biomedical Innovation, Health and Nutrition
Developer Osamu Suzuki
Depositor Osamu Suzuki
Conditions of distribution Collaboration with the depositor only. It's necessary to obtain depositor's consent on use of biological resources. contact us
Animal Health Report
Strain description Comparison of coat colors

A mouse strain in which Hps5, one of the genes responsible for Hermansky-Pudlak syndrome (HPS), was knocked out by genome editing (CRISPR/Cas9). It was created by using two gRNAs targeting Exon 2 and Exon 7 of the Hps5 gene simultaneously and deleting about 7.4 kb between them. Homozygous KO mice have a paler coat color than C57BL/6N wild-type mice (B6 mice, see photos). Western blotting confirmed the disappearance of normal-sized proteins in the kidney, spleen, and testis. The strain is expected to be used as an HPS model.

Reference(s)
Resource information
Cryopreserved sperm In-house Genotype
Medium
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types Knockout
Gene symbol Hps5
Gene name biogenesis of lysosomal organelles complex 2 subunit 2, Hermansky-Pudlak syndrome 5, ru2, ru-2, ruby eye 2
Genotyping information Genotyping protocol for Hps5-KO mice (pdf).
html>