HOME > mice > Hps5-KO mice
Strain Detail: Hps5-KO mice
| Strain information |
| Resource No. |
nbio337 |
| Strain name |
Hps5-KO |
| Strain Nomenclature |
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| Common name/Synonyms |
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| Strain types |
mutant |
| Background strain |
C57BL/6N |
| Institution |
Laboratory of Animal Models for Human Diseases, National Institutes of Biomedical Innovation, Health and Nutrition |
| Developer |
Osamu Suzuki |
| Depositor |
Osamu Suzuki |
| Conditions of distribution |
Collaboration with the depositor only. It's necessary to obtain depositor's consent on use of biological resources. contact us |
| Animal Health Report |
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| Strain description |
 A mouse strain in which Hps5, one of the genes responsible for Hermansky-Pudlak syndrome (HPS), was knocked out by genome editing (CRISPR/Cas9). It was created by using two gRNAs targeting Exon 2 and Exon 7 of the Hps5 gene simultaneously and deleting about 7.4 kb between them. Homozygous KO mice have a paler coat color than C57BL/6N wild-type mice (B6 mice, see photos). Western blotting confirmed the disappearance of normal-sized proteins in the kidney, spleen, and testis. The strain is expected to be used as an HPS model. |
| Reference(s) |
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| Resource information |
| Cryopreserved sperm |
In-house |
Genotype |
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| Medium |
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| From other institutions |
Genotype |
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| Medium |
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| Cryopreserved embryo |
In-house |
Mating System |
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| Genotype |
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| Medium |
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| From other institutions |
Mating System |
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| Genotype |
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| Method |
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| Strain status / Availability |
Cryopreserved sperm |
Within 1 month |
| Cryopreserved embryo |
Within 1 month |
| Live animals |
Approx. 2 months |
| Gene information |
| Mutation types |
Knockout |
| Gene symbol |
Hps5 |
| Gene name |
biogenesis of lysosomal organelles complex 2 subunit 2, Hermansky-Pudlak syndrome 5, ru2, ru-2, ruby eye 2 |
| Genotyping information |
Genotyping protocol for Hps5-KO mice (pdf). |
