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Strain Detail: LKB1-DN/B mice

Strain information
Resource No. nbio075
Strain name LKB1-DN/B
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain C57BL/6J
Institution Department of Food Nutritional Science, University of Shizuoka
Developer Shinji Miura
Depositor Shinji Miura
Conditions of distribution

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Animal Health Report
Strain description

LKB1 is an enzyme regulating the activity of AMP-activated protein kinase (AMPK) family. LKB1-DN mice are transgenic mouse lines with skeletal-muscle-specific overexpression of a dominant negative form of LKB1. The overexpression suppresses the LKB1 activity in the skeletal muscle. Comparison of LKB1-DN mice with AMPK-DN mice (nbio079-082), which show skeletal-muscle-specific suppression of AMPK, and with wild-type mice indicated that LKB1-DN mice showed decrease in endurance during exercise in addition to decreased O2intake and CO2excretion. These observation suggests that LKB1 exerts AMPK-independent effect on the diaphragm function and is involved in endurance during exercise, and also in O2intake. There are two LKB1-DN lines (LKB1-DN/B and LKB1-DN/C) with no apparent difference between them.

  1. Miura S, Kai Y, Tadaishi M, Tokutake Y, Sakamoto K, Bruce CR, Febbraio MA, Kita K, Chohnan S, and Ezaki O. (2013) Marked phenotypic differences of endurance performance and exercise-induced oxygen consumption between AMPK and LKB1 deficiency in mouse skeletal muscle: changes occurring in the diaphragm. Am. J. Physiol. Endocrinol. Metab. 305(2):E213-29. [PMID:23695215]
  2. http://dfns.u-shizuoka-ken.ac.jp/labs/nutrbioc/index3.html/Research7.html (in Japanese)
Resource information
Cryopreserved sperm In-house Genotype
From other institutions Genotype
Cryopreserved embryo In-house Mating System
Medium EFS40
From other institutions Mating System
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol Stk11 (Lkb1)
Gene name serine/threonine kinase 11
Genotyping information