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Strain Detail

Strain information
Resource No. nbio076
Strain name LKB1-DN/C
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain C57BL/6J
Institution Department of Food Nutritional Science, University of Shizuoka
Developer Shinji Miura
Depositor Shinji Miura
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

LKB1 is an enzyme regulating the activity of AMP-activated protein kinase (AMPK) family. LKB1-DN mice are transgenic mouse lines with skeletal-muscle-specific overexpression of a dominant negative form of LKB1. The overexpression suppresses the LKB1 activity in the skeletal muscle. Comparison of LKB1-DN mice with AMPK-DN mice (nbio079-082), which show skeletal-muscle-specific suppression of AMPK, and with wild-type mice indicated that LKB1-DN mice showed decrease in endurance during exercise in addition to decreased O2 intake and CO2 excretion. These observations suggest that LKB1 exerts AMPK-independent effect on the diaphragm function and is involved in endurance during exercise, and also in O2 intake. There are two LKB1-DN lines (LKB1-DN/B and LKB1-DN/C) with no apparent difference between them.

References
  1. Miura S, Kai Y, Tadaishi M, Tokutake Y, Sakamoto K, Bruce CR, Febbraio MA, Kita K, Chohnan S, Ezaki O. Marked phenotypic differences of endurance performance and exercise-induced oxygen consumption between AMPK and LKB1 deficiency in mouse skeletal muscle: changes occurring in the diaphragm. Am J Physiol Endocrinol Metab. 2013 Jul 15;305(2):E213-29.
  2. http://dfns.u-shizuoka-ken.ac.jp/labs/nutrbioc/index3.html/Research7.html (in Japanese)
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol Stk11 (Lkb1)
Gene name serine/threonine kinase 11
Genotyping information