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Strain Detail

Strain information
Resource No. nbio022
Strain name C57BL/6-Dll3oma
Strain Nomenclature
Common name/Synonyms oma mice
Strain types congenic
Background strain
Institution Graduate School of Natural Science and Technology, Okayama University
Developer Tetsuo Kunieda
Depositor Tetsuo Kunieda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description Photo of oma mice

The oma mice are skeletal anomaly mutant found in 4 way cross strain derived from C57BL/6J, C3H, DBA/2 and BALB/c maintained in School of Agriculture, Okayama University. These mice observe remarkable deformity and fusion of vertebrae, and deformity and bifurcation of ribs. It shows that phenotype of oma mice causes to change 409th glycine residue to cysteine by single base substitution occurred on 8th exon of Delta-like3 (Dll3) gene. C57BL/6-Dll3oma is a congenic strain by 17 times backcrossing to C57BL/6J with original oma mice.

Genotyping

Genotyping of Dll3oma is able to carry out following PCR-RFLP. Folloing two primer is used for PCR. Forward primer was designed as mismatch primer by substituting CAGC at the 3'-terminal for CGGC derived from original genome in order that transgene is digested by PvuII.

  • Primer F: 5'-AACGGCGGCACGTGCGTGGAGGGCAGC-3'
  • Primer R: 5'-CGTCCGGGCGCACAGCGAACTCGCATC-3'

PCR carry out following conditions.

  • 95C: 3 min
  • (95C: 30 sec, 60C: 30 sec, 72C: 30 sec ) x 40 cycles
  • 72C: 10 min

Two genotypes are discriminated by agarose gel electrophoresis after PCR productions are digested by restriction enzyme PvuII.

  • wildtype (+) : 211bp
  • transgene (Dll3oma) : 185bp (+26bp)
References
  1. Shinkai. Y., Tsuji. T., Kawamoto. Y., and Kunieda. T.(2004) New mutant mouse with skeletal deformities caused by mutation in Delta like 3(Dll3) gene. Exp. Anim. 53(2) 129-136.
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types spontaneus mutation
Gene symbol Dll3
Gene name Delta-like 3
Genotyping information