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Strain Detail

Strain information
Resource No. nbio186
Strain name PKAchu-NC
Strain Nomenclature ICR×(ICR×B6C3F1)N2-Tg(pT2A-3906NES)#1
Common name/Synonyms AKAREV-NC mice
Strain types mutant
Background strain
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

PKAchu-NC mice are transgenic mice expressing förster (or fluorescence) resonance energy transfer (FRET) biosensor AKAR3EV-NC under the control of CAG promoter in whole body. AKAR3EV-NC, which is muteted in phosphorylation site on the substrate of cytoplasm-localized FRET biosensor AKAR3EV monitoring PKA kinase activity, is possible to use as the negative control of every cytoplasm-localized FRET biosensor, including AKAR3EV or EKAREV.

References
  1. Kamioka Y, Sumiyama K, Mizuno R, Sakai Y, Hirata E, Kiyokawa E, Matsuda M. Live Imaging of Protein Kinase Activities in Transgenic Mice Expressing FRET Biosensors. Cell Struct Funct. 2012 Jan 24. [Epub ahead of print] PMID: 22277578
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol CFP, YFP
Gene name Cyan Fluorescent Protein, Yellow Fluorescent Protein
Genotyping information