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Strain Detail: PKAchu-NC mice
| Strain information |
| Resource No. |
nbio186 |
| Strain name |
PKAchu-NC |
| Strain Nomenclature |
ICR×(ICR×B6C3F1)N2-Tg(pT2A-3906NES)#1 |
| Common name/Synonyms |
AKAREV-NC mice |
| Strain types |
mutant |
| Background strain |
|
| Institution |
Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University |
| Developer |
Michiyuki Matsuda |
| Depositor |
Michiyuki Matsuda |
| Conditions of distribution |
It's necessary to obtain depositor's consent on use of biological resources. contact us |
| Animal Health Report |
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| Strain description |
PKAchu-NC mice are transgenic mice expressing förster (or fluorescence) resonance energy transfer (FRET) biosensor AKAR3EV-NC under the control of CAG promoter in whole body. AKAR3EV-NC, which is muteted in phosphorylation site on the substrate of cytoplasm-localized FRET biosensor AKAR3EV monitoring PKA kinase activity, is possible to use as the negative control of every cytoplasm-localized FRET biosensor, including AKAR3EV or EKAREV. |
| Reference(s) |
- Kamioka Y, Sumiyama K, Mizuno R, Sakai Y, Hirata E, Kiyokawa E, and Matsuda M. (2012) Live imaging of protein kinase activities in transgenic mice expressing FRET biosensors. Cell Struct Funct 37(1):65-73. [PMID:22277578]
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| Resource information |
| Cryopreserved sperm |
In-house |
Genotype |
|
| Medium |
FERTIUP |
| From other institutions |
Genotype |
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| Medium |
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| Cryopreserved embryo |
In-house |
Mating System |
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| Genotype |
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| Medium |
EFS40 |
| From other institutions |
Mating System |
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| Genotype |
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| Method |
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| Strain status / Availability |
Cryopreserved sperm |
Within 1 month |
| Cryopreserved embryo |
Within 1 month |
| Live animals |
Approx. 2 months |
| Gene information |
| Mutation types |
transgenic |
| Gene symbol |
CFP, YFP |
| Gene name |
Cyan Fluorescent Protein, Yellow Fluorescent Protein |
| Genotyping information |
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