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Strain Detail

Strain information
Resource No. nbio021
Strain name loxP-Rap1GAPII-Tg
Strain Nomenclature
Common name/Synonyms RapGAP mice
Strain types mutant
Background strain C57BL/6Cr Slc
Institution National Cerebral and Cardiovascular Center
Developer Naoki Mochizuki
Depositor Naoki Mochizuki
Conditions of distribution Only for collaboration with the depositor : contact us
Animal Health Report
Strain description

The loxP-Rap1GAPII-Tg mice are transgenic mice overexpressing FLAG-Tagged human Rap1GAPII. This strain were produced using pCAGNL5 vector and CAG promoter. This strain expresses FLAG-Tagged human Rap1GAPII by mating with Cre expressing mice. Rap1GAPII inhibits low molecular G-protein rap1 activity.

References
  1. Mochizuki N, Ohba Y, Kiyokawa E, Kurata T, Murakami T, Ozaki T, Kitabatake A, Nagashima K, Matsuda M. Activation of the ERK/MAPK pathway by an isoform of rap1GAP associated with Gαi. Nature. 1999 Aug 26;400(6747): 891-894.
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol Garnl4 (Rap1Gap2)
Gene name GTPase activating RANGAP domain-like 4 (RAP1 GTPase activating protein 2)
Genotyping information