HOME > mice > TBK1, IKKi, TNF KO mice

Strain Detail

Strain information
Resource No. nbio156
Strain name TBK1, IKKi, TNF KO
Strain Nomenclature
Common name/Synonyms IKKi (Ikbke) mice
Strain types mutant
Background strain
Institution Research Institute for Microbial Diseases, Osaka University
Developer Shizuo Akira
Depositor Shizuo Akira
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. In publishing the research results obtained by use of TNF KO mice, a citation of the following literature (ref. 3) designated by the depositor and a mention of using C57BL/6 congenic mice in the article is requested. : contact us
Animal Health Report
Strain description

TANK-binding kinase 1 (TBK1) and inducible IκB kinase (IKKi) are the menber of IKK family and signal transduction molecules in the downstream of retinoic acid inducible gene-1 (RIG-1) family, TLR3 and TLR4. It's essensial for the production of interferon type I. These gene KO mice are useful for the elucidation of mechanism for innate immunity activation by infection of pathogen or DNA vaccine. TBK1 KO mice are embryonic lethal and TBK1 KO/TNF KO double KO mice are survive. Therefore, we cryopreserved embryos produced by IVF between IKKi+/- TBK1-/- TNF-/- or IKKi-/- TBK1+/- TNF-/- male and C57BL/6J female. IKKi/TBK1/TNF triple KO mice are also embryonic lethal. Please contact us if you want to be supplied.

References
  1. Koyama S, Ishii KJ, Kumar H, Tanimoto T, Coban C, Uematsu S, Kawai T, Akira S. Differential role of TLR- and RLR-signaling in the immune responses to influenza A virus infection and vaccination. J Immunol. 2007 Oct 1;179(7):4711-20. [PMID: 17878370]
  2. Hemmi H, Takeuchi O, Sato S, Yamamoto M, Kaisho T, Sanjo H, Kawai T, Hoshino K, Takeda K, Akira S. The roles of two IκB kinase-related kinases in lipopolysaccharide and double stranded RNA signaling and viral infection. J Exp Med. 2004 Jun 21;199(12):1641-50. [PMID: 15210742]
  3. Taniguchi T, Takata M, Ikeda A, Momotani E, Sekikawa K. Failure of germinal center formation and impairment of response to endotoxin in tumor necrosis factor α-deficient mice. Lab Invest. 1997 Dec;77(6):647-58. [PMID: 9426403]
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types knockout
Gene symbol Tbk1 (1200008B05Rik), Ikki (Ikbke, IKKepsilon), Tnf (DIF, TNF α, TNF-α, Tnfa, TNFα, Tnfsf1a, tumor necrosis factor-α)
Gene name TANK-Binding Kinase 1, Inducible IκB kinase, Tumor necrosis factor
Genotyping information PCR protocol for genotyping Tbk1-ko alleles : pdf file