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Strain Detail: TH-Luc 177

Strain information
Resource No. nbio308
Strain name TH-Luc 177
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain C57BL/6J Jcl
Institution Nara Institute of Science and Technology
Developer Mitsuko Ishihara-Sugano
Depositor Mitsuko Ishihara-Sugano
Conditions of distribution

In publishing the research results obtained by use of the biological resource, a citation of the following literature (ref. 1) is requested. It's necessary to obtain depositor's consent on use of biological resources. Contact us

Animal Health Report
Strain description

The toxicity of dioxins and related compounds is mediated mainly by aryl hydrocarbon receptor (AhR) signaling. A binding motif of AhR, the AhR-responsive element III (AHRE-III), is located upstream from the gene encoding tyrosine hydroxylase (TH), the rate limiting enzyme of dopamine biosynthesis. The transgenic mice, Th-Luc, were produced with inserted constructs of the AHRE-III enhancers, TH gene promoter and the c-myc-tagged luciferase gene (The constructs are shown in a figure bellow). Using these mice, the effect of exposure of AhR activating agents (e.g., dioxins, PCBs, and benzpyrene) can be examined on midbrain dopaminergic neurons.

Three sublines (176, 177, and 183 strains) are available (different in the transgene copy numbers). TH-Luc 176 strain was used in the literature.

Structure of the transgene

Reference
  1. Tanida T, Tasaka K, Akahoshi E, Ishihara-Sugano M, Saito M, Kawata S, Danjo M, Tokumoto J, Mantani Y, Nagahara D, Tabuchi Y, Yokoyama T, Kitagawa H, Kawata M, and Hoshi N. (2014) Fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin transactivates aryl hydrocarbon receptor-responsive element III in the tyrosine hydroxylase immunoreactive neurons of the mouse midbrain. J Appl Toxicol 34(2):117-26. [PMID:23296914]
Resource information
Cryopreserved sperm In-house Genotype
Medium
From other institutions Genotype Tg/+ (Hemizygous)
Medium
Cryopreserved embryo In-house Mating System
Genotype Tg/+ (Hemizygous)
Medium DAP213
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol Th
Gene name Mouse tyrosine hydroxylase enhancer/promoter, fire fly luciferase, SV40 PolyA,c-myc-tag
Genotyping information