HOME > mice > C3G KO mice
Strain Detail: C3G KO mice
| Strain information |
| Resource No. |
nbio112 |
| Strain name |
C3G KO |
| Strain Nomenclature |
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| Common name/Synonyms |
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| Strain types |
mutant |
| Background strain |
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| Institution |
Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University |
| Developer |
Michiyuki Matsuda |
| Depositor |
Michiyuki Matsuda |
| Conditions of distribution |
In publishing the research results obtained by use of the biological resource, a citation of the following literature (ref. 1) designated by the depositor is requested. contact us |
| Animal Health Report |
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| Strain description |
C3G is an activator of Rap1, which is a low molecular weight GTP binding protein to belong to Ras family playing an important role of signal transduction, and it's GDP-GTP exchange factor or guanine nucleotide exchange factor (GEF). C3G KO mice is embryonic lethal before embryonic day 7.5. The lethality is rescued by the expression of the human C3G transgene (hC3G Tg mice, nbio113), but it's incomplete. Consequently, C3G homozygous knockout mouse introduced human C3G transgene (C3G-/-/hu) is remarkably small. It's showed that C3G is essential for cell adhesion and flattening by the research of C3G transgene deleted cells produced by expressing Cre in embryonic fibroblast cells derived from C3G-/-/hu mouse. It's possible to supply C3G heterozygous knockout mice. |
| Reference(s) |
- Ohba Y, Ikuta K, Ogura A, Matsuda J, Mochizuki N, Nagashima K, Kurokawa K, Mayer BJ, Maki K, Miyazaki J, and Matsuda M. (2001) Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis. EMBO J. 20(13):3333-41. [PMID:11432821]
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| Resource information |
| Cryopreserved sperm |
In-house |
Genotype |
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| Medium |
FERTIUP |
| From other institutions |
Genotype |
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| Medium |
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| Cryopreserved embryo |
In-house |
Mating System |
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| Genotype |
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| Medium |
EFS40 |
| From other institutions |
Mating System |
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| Genotype |
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| Method |
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| Strain status / Availability |
Cryopreserved sperm |
Within 1 month |
| Cryopreserved embryo |
Within 1 month |
| Live animals |
Approx. 2 months |
| Gene information |
| Mutation types |
knockout |
| Gene symbol |
Rapgef1 (C3G, Grf2) |
| Gene name |
Rap guanine nucleotide exchange factor (GEF) 1 (C3G, Guanine nucleotide releasing factor 2) |
| Genotyping information |
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