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Strain Detail

Strain information
Resource No. nbio113
Strain name hC3G Tg
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain
Institution Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution In publishing the research results obtained by use of the biological resource, a citation of the following literature (ref. 1) designated by the depositor is requested. : contact us
Animal Health Report
Strain description

C3G is an activator of Rap1, which is a low molecular weight GTP binding protein to belong to Ras family playing an important role of signal transduction, and it's GDP-GTP exchange factor or guanine nucleotide exchange factor (GEF). The hC3G Tg mice overexpress human C3G in whole body and CAG promoter. This strain is available for C3G conditional Tg/KO because it's able to delete C3G transgene by Cre/loxP system. The embryonic lethality of C3G KO mice (nbio112) can be rescued by using hC3G Tg mice, but it's incomplete. Consequently, C3G homozygous knockout mouse introduced human C3G transgene (C3G-/-/hu) is remarkably small. It's showed that C3G is essential for cell adhesion and flattening by the research of C3G transgene deleted cells produced by expressing Cre in embryonic fibroblast cells derived from C3G-/-/hu mouse.

References
  1. Ohba Y, Ikuta K, Ogura A, Matsuda J, Mochizuki N, Nagashima K, Kurokawa K, Mayer BJ, Maki K, Miyazaki J, Matsuda M. Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis. EMBO J, 20: 3333-3341, 2001. [PMID: 11432821]
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types knockout
Gene symbol Rapgef1 (C3G, Grf2)
Gene name Rap guanine nucleotide exchange factor (GEF) 1 (C3G, Guanine nucleotide releasing factor 2)
Genotyping information