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Strain Detail

Strain information
Resource No. nbio135
Strain name ADAMTS13S/S
Strain Nomenclature 129+Ter/SvJcl-Adamts13S/S NCVC
Common name/Synonyms
Strain types congenic
Background strain
Institution National Cerebral and Cardiovascular Center Research Institute
Developer Toshiyuki Miyata
Depositor Toshiyuki Miyata (Contact person: Koichi Kokame)
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. : contact us
Animal Health Report
Strain description

ADAMTS13S/S mice is established to introduce Adamts13 gene derived from C57BL/6 strain into 129/Sv strain by backcrossing. These mice express C-terminal domain deleted ADAMTS13 (ADAMTS13S) by the insertion of retrotransposon into Adamts13 gene., whereas wildtype 129/Sv mice express full-length ADAMTS13 (ADAMTS13L).

These mice are remarkable grade of thrombocytopenia caused by experimental collagen administration compared with ADAMTS13L expressed mice (wildtype 129/Sv), not so much as ADAMTS13 KO mice (nbio061, nbio074). Therefore, it's considered that C-terminal domain deletion of ADAMTS13 gives potential thrombosis risk to mice.

It's known that ADAMTS13 is a plasma protease which cleaves von Willebrand factor (VWF) specifically and deletion of ADAMTS13 activity is caused thrombotic thrombocytopenic purpura (TTP) in human.

ADAMTS13L/L mice (nbio134) which are introduced Adamts13 gene derived from 129/Sv strain into C57BL/6 strain by backcrossing and express full-length ADAMTS13 (ADAMTS13L) is also available.

References
  1. Banno F, Chauhan AK, Kokame K, Yang J, Miyata S, Wagner DD, Miyata T: The distal carboxyl-terminal domains of ADAMTS13 are required for regulation of in vivo thrombus formation. Blood, 113(21):5323-5329 (2009). [PMID:19109562]
  2. Banno F, Kaminaka K, Soejima K, Kokame K, Miyata T: Identification of strain-specific variants of mouse Adamts13 gene encoding von Willebrand factor-cleaving protease. J Biol Chem, 279, 30896-30903 (2004). [PMID:15136581]
Resource information
Cryopreserved sperm In-house Genotype
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System
Genotype
Medium EFS40
From other institutions Mating System
Genotype
Medium
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live mouse
Approx. 2 months
Gene information
Mutation types
Gene symbol Adamts13
Gene name A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 13
Genotyping information