Strain Detail: PGC-1α-b/A-line mice

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Three mRNA isoforms (PGC-1α-a, -b, and -c) are transcribed from PGC-1α (Ppargc1a) gene. Slightly different proteins are translated from the three mRNA isoforms. Expression of each isoform is different depending on tissue types and stimuli. Exercise increases PGC-1α-b and PGC-1α-c mRNAs, but not PGC-1α-a mRNA, in the skeletal muscle. On the other hand, fasting increased PGC-1α-a mRNA, but not PGC-1α-b or PGC-1α-c mRNAs, in the liver.

The PGC-1α-b/A-line is a transgenic mouse line with skeletal-muscle-specific (driven by human α-actin promotor) overexpression of PGC-1α-b mRNA (derived from the line 02-1 in Table 1 of Ref. 1). This strain is useful for studying isoform-specific functions of PGC-1α mRNAs and proteins in skeletal muscles.

Multiple sublines overexpressing each isoform in skeletal muscles are available: three sublines (nbio095, nbio096, and nbio097) for PGC-1α-a, two sublines (nbio299, and nbio300) for PGC-1α-b, and three sublines (nbio301, nbio302, and nbio303) for PGC-1α-c.

1. Miura S, Kai Y, Kamei Y, and Ezaki O. (2008) Isoform-specific increases in murine skeletal muscle peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) mRNA in response to β2-adrenergic receptor activation and exercise. Endocrinology 149(9):4527-33. [PMID:18511502]
2. Tadaishi M, Miura S, Kai Y, Kano Y, Oishi Y, and Ezaki O. (2011) Skeletal muscle-specific expression of PGC-1α-b, an exercise-responsive isoform, increases exercise capacity and peak oxygen uptake. PLoS ONE 6(12):e28290. [PMID:22174785]