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Strain Detail: PGC-1α-b/B-line

Strain information
Resource No. nbio300
Strain name PGC-1α-b/B-line
Strain Nomenclature
Common name/Synonyms
Strain types mutant
Background strain C57BL/6N
Institution Department of Food Nutritional Science, University of Shizuoka
Developer Shinji Miura
Depositor Shinji Miura
Conditions of distribution

Only for collaboration with the depositor : contact us

Animal Health Report
Strain description

Three mRNA isoforms (PGC-1α-a, -b, and -c) are transcribed from PGC-1α (Ppargc1a) gene. Slightly different proteins are translated from the three mRNA isoforms. Expression of each isoform is different depending on tissue types and stimuli. Exercise increases PGC-1α-b and PGC-1α-c mRNAs, but not PGC-1α-a mRNA, in the skeletal muscle. On the other hand, fasting increased PGC-1α-a mRNA, but not PGC-1α-b or PGC-1α-c mRNAs, in the liver.

The PGC-1α-b/B-line is a transgenic mouse line with skeletal-muscle-specific (driven by human α-actin promotor) overexpression of PGC-1α-b mRNA (derived from the line 03-2 in Table 1 of Ref. 1). This strain is useful for studying isoform-specific functions of PGC-1&alpha mRNAs and proteins in skeletal muscles.

Multiple sublines overexpressing each isoform in skeletal muscles are available: three sublines (nbio095nbio096nbio097) for PGC-1α-a, two sublines (nbio299nbio300) for PGC-1α-b, and three sublines (nbio301nbio302nbio303) for PGC-1α-c.

References
  1. Miura S, Kai Y, Kamei Y, and Ezaki O. (2008) Isoform-specific increases in murine skeletal muscle peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) mRNA in response to β2-adrenergic receptor activation and exercise. Endocrinology 149(9):4527-33. [PMID:18511502]
  2. Tadaishi M, Miura S, Kai Y, Kano Y, Oishi Y, and Ezaki O. (2011) Skeletal muscle-specific expression of PGC-1α-b, an exercise-responsive isoform, increases exercise capacity and peak oxygen uptake. PLoS ONE 6(12):e28290. [PMID:22174785]
Resource information
Cryopreserved sperm In-house Genotype Heterozygous
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System IVF
Genotype mix of KO/+ and wild-type
Medium EFS40
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types transgenic
Gene symbol Ppargc1a (PGC-1α)
Gene name Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Genotyping information